Sensorless Wavefront Correction in Two-Photon Microscopy Across Different Turbidity Scales

Adaptive optics (AO) is a powerful tool to increase the imaging depth of multiphoton scanning microscopes. For highly scattering tissues, sensorless wavefront correction techniques exhibit robust performance and present straight-forward implementation AO. However, for many applications such as live-tissue imaging, speed aberration remains critical bottleneck. Dynamic Scattering compensation Holography (DASH) -- fast-converging AO technique introduced recently scatter in nonlinear microscopy addresses this issue. DASH has been targeted at turbid media, but to-date it remained an open question how performs mild turbidity, where limitations imposed by phase-only shaping are expected impede its convergence. In work, we study across different turbidity regimes, simulation well experiments. We further provide direct comparison between novel, modified version Continuous Sequential Algorithm (CSA) which call Amplified CSA (a-CSA).